Isolation and Characterization of Plasma Membranes from Human Leukemic Lymphocytes1
نویسنده
چکیده
A method for the isolation of lymphocyte plasma mem branes from patients with chronic lymphocytic leukemia is described. Lymphocytes were disrupted in a hypotonie bicarbonate medium using a Dounce homogenizer. The plasma membranes fraction was finally collected from a continuous sucrose gradient at density 1.115 (g/ml). Elec tron micrographs of this material showed membranes and small dense vesicles of unidentified origin. The enrichment of this fraction in adenosine 5'-monophosphatase, Mg2+:Na+:K+:adenosine triphosphatase, and uridine di-
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تاریخ انتشار 2006